【Test Purpose】
Canine Cortisol (cCortisol) is a steroid hormone produced by the canine adrenal
cortex. Cortisol helps maintain a constant blood sugar level, reduces inflammation
and helps the body manage stress. While various conditions caused by abnormally
high levels of the cortisol are called Cushing syndrome (CS), both dogs and cats can
suffer from CS, which is more common in dogs than in cats. Dogs of middle and old
age (about 7 to 12 years old) are most likely to develop the disease. The disease
develops slowly and early symptoms are not easy to detect. CS can be clinically
diagnosed by adrenocorticotropic hormone (ACTH) stimulation test and dexam
ethasone suppression test, and its different types: adrenocortical tumors hyperadr
enocorticism(ATH)and pituitary-dependent hyperadrenocorticism(PDH).
【Main Ingredients
【Detection principle】
This product uses fluorescence immunochromatography to quantitatively detect
the content of cCortisol in dog serum/plasma. Basic principle: T and C lines are
marked on the nitrocellulose membrane, the T line is coated with cCortisol antigen
a, and the binding pad is sprayed with a fluorescent nanomaterial labeled antibody
b that can specifically recognize cCortisol. The cCortisol in the sample is first
labeled with the nanomaterial. The antibody b binds to form a complex, and then
chromatographs upward. The complex competes with T-line antigen a and cannot
be captured; On the contrary, when there is no cCortisol in the sample, antibody
b binds to antigen a. When the excitation light is irradiated, the nano material
emits a fluorescent signal, and the strength of the signal is inversely proportional to
the concentration of cCortisol in the sample.
1. cCortisol test reagent card
2. Sample diluent
3. Tip
4. Instructions etc.